DNA

Part:BBa_K2100009:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-10)


pENTR pPRE4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 278
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 278
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 278
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 278
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 19
    Illegal BsaI.rc site found at 265


Design Notes

This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This is a synthetic promoter from IDT.

References